Journal: BioMed Research International

Article Title: Proteomic Analysis of Fetal Ovaries Reveals That Primordial Follicle Formation and Transition Are Differentially Regulated

doi: 10.1155/2017/6972030

Figure Lengend Snippet: Representative 2D-DIGE gel image of differentially expressed proteins of fetal ovaries at day 55 and day 90 of gestation. The proteins extracted from the fetal ovaries at day 55 and day 90 of gestation samples were labelled with cy3 and cy5, respectively. An internal standard protein sample (a mixture of fetal ovaries at day 55 and day 90 of gestation samples) was labelled with the Cy2 dye. The number in the figure corresponds to the number shown in .

Article Snippet: The 2D DIGE gel images were analyzed by the Image Master 2D platinum 7.0 software (GE Healthcare Life Sciences, NJ, USA) and the protein abundance changes for spot picking detection were calculated using cy3/cy2 and cy5/cy2 differential in-gel analysis ratios.

Techniques:

Journal: Macromolecules

Article Title: Preservation of Photoluminescence Efficiency in the Ordered phases of Poly(2,3-diphenyl-1,4-phenylenevinylene) via Disturbing the Intermolecular π–π Interactions with Dendritic Aliphatic Side Chains

doi: 10.1021/ma300640n

Figure Lengend Snippet: Figure 4. Set of 1D WAXD patterns of (a) P1, (b) P2 and (c) P3. The bottom patterns in each figure are the 1D WAXD patterns of the dried samples recorded at 30 °C. The top patterns are the 1D WAXD patterns of the thermally treated samples, which were cooled from the I phases to room temperature, and recorded at 30 °C.

Article Snippet: Two-dimensional (2D) WAXD patterns were obtained using a Bruker D8Discover diffractometer with GADDS as a 2D detector.

Techniques:

Journal: Macromolecules

Article Title: Preservation of Photoluminescence Efficiency in the Ordered phases of Poly(2,3-diphenyl-1,4-phenylenevinylene) via Disturbing the Intermolecular π–π Interactions with Dendritic Aliphatic Side Chains

doi: 10.1021/ma300640n

Figure Lengend Snippet: Figure 5. 2D WAXD patterns of the ordered phases of (a) P1, (b) P2, and (c) P3 after shearing. Solid arrow indicates the direction of mechanical shearing force applied on the sample. The incident X-ray beam was along the normal direction of the 2D patterns. Schematic representations of the molecular dimensions along the chain and the lateral directions are illustrated in (d) for P1, (e) for P2, and (f) for P3, respectively.

Article Snippet: Two-dimensional (2D) WAXD patterns were obtained using a Bruker D8Discover diffractometer with GADDS as a 2D detector.

Techniques:

Journal: BMC Plant Biology

Article Title: Integration of proteomic and genomic approaches to dissect seed germination vigor in Brassica napus seeds differing in oil content

doi: 10.1186/s12870-018-1624-7

Figure Lengend Snippet: The 2D-DIGE maps and the functional categorization of DEPs in germinating B. napus seeds with high and low oil content. a - e represent the 2D-DIGE maps of 12WH191 (H) and KenC8 (L) germinating seeds at 0, 12, 24, 36 and 48 HAI. f represents the functional categorization of DEPs. Arrows show the protein spots that were highly expressed in low oil-containing seeds; lines show the protein spots that were highly expressed in high oil-containing seeds

Article Snippet: For 2D-DIGE analysis, the labeled proteins were mixed with 2D-DIGE buffer (7 M urea, 2 M thiourea, 4% CHAPS, 0.4% DTT, 0.5% IPG buffer) and separated with isoelectric focusing after being loaded on an immobilized pH gradient strip (IPG, pH 4–7, 24 cm; Amersham Biosciences, Uppsala, Sweden) [ ].

Techniques: Functional Assay